Fig. 4

Altered hypercapnic CBF and CVR in zQ175DN HET mice at 15 months of age. (A) Mean midbrain absolute CBF maps under baseline, under CO₂ Challenge and CVR in both WT and zQ175DN HET group and a statistical T-map from VBA for each condition, overlaid on the study-specific template, showing local genotypic differences (Two-sample T-test, p < 0.05, FDR corrected, k ≥ 10); (B) RBA in predefined cortical and subcortical regions under baseline, CO₂ Challenge and CVR, represented with subject values of absolute CBF and a group mean ± SD (multiple two-sample T-test, p < 0.05, FDR corrected); Significant difference after FDR correction * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001; CgCtx—Cingulate Cortex, Cl – Claustrum, InsCtx – Insular Cortex, M1/2Ctx – Motor Cortex 1/2, S1/2Ctx – Somatosensory Cortex 1/2, CPu – Caudate Putamen, GP – Globus Pallidus