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Fig. 4 | Journal of Biomedical Science

Fig. 4

From: MiR-30a and miR-379 modulate retinoic acid pathway by targeting DNA methyltransferase 3B in oral cancer

Fig. 4

The effects of miR-30a and miR-379 overexpression on OSCC cells. a qRT–PCR analysis showing the expression level of miR-30a (pLemiR-30a) and miR-379 (pLemiR-379) compared with vector control (pLemiR-NS) in SCC-15 and OEC-M1 cells. b Colony formation assay after miR-30a and miR-379 transfection in SCC-15 and OEC-M1 cells for 7 days (left). The mean number of colonies for each well was determined from three independent assays (right). c Growth rates of OEC-M1 cells measured by MTT assay after vector control, miR-30a or miR-379 transfection. d OEC-M1 cells co-transfected with 1 μg of empty control vector and pRXR vector were incubated with the vehicle control (DMSO, 10 nM), 9-cis-RA (25 nM), control mimics (NC, 20 nM), miR-30a (20 nM) or miR-379 (20 nM). The relative luciferase activity of each sample is measured at 48 h after transfection and normalized to Renilla luciferase activity. e ChIP assay of ADHFE1 and ALDH1A2 promoter region was performed with OEC-M1 cells using anti-DNMT3B antibody, anti-acetyl-histone H3 (H3Ac) antibody, anti-histone H3 trimethylation of lysine 9 (H3K9me3) antibody, anti-histone H3 trimethylation of lysine 27 (H3K27me3) antibody, control mouse IgG (mIgG) antibody and control rabbit IgG (rIgG) antibody after treatment with control mimics (NC, 20 nM), or miRNA mimics (PM-30a or PM-379, 20 nM) for 48 h. All data are presented as mean ± SD; **p < 0.01; ***p < 0.001

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