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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: Transgenic expression of omega-3 PUFA synthesis genes improves zebrafish survival during Vibrio vulnificus infection

Fig. 1

Transgenic zebrafish specifically express Fadsd6 and Elvol5a in liver. (a) Schematics of the liver-specific activator plasmid pT2-LF-Tta (containing an activator (tTA) driven by the zebrafish fabp10 promoter) and two tetracycline-responsive plasmids (pT2-TRE-fadsd6-TCFP11 and pT2-TRE-elvol5a-TCFP13). All expression cassettes are flanked by the Tol2 transposon. (b) Fluorescence microscopy images of F3 Fadsd6 (red) and Elvol5a (green) transgenic zebrafish larvae, taken at five days (5D) after hatching. (c) RT-PCR was used to detect exogenous gene expression of Fadsd6 and Elvol5a in adult transgenic and Wt zebrafish. (d) Western blots were performed to detect fusion of the V5-tag to Fadsd6 and Elvol5a in transgenic and Wt zebrafish. Mouse β-actin was used as a positive control

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