Fig. 1
From: Activation of MEK2 is sufficient to induce skin papilloma formation in transgenic zebrafish
a. Phylogenetic analysis of the MEK gene family in the human, mouse, and zebrafish. Each member of the MEK family was separated into individual monophyletic groups. Amino acid sequences were aligned with ClustalW. Accession numbers for these sequences are as follows: zMEK1 (NP_998584), hMEK1 (NP_002746), mMEK1 (NP_032953), zMEK2 (zMEK2a, NP_001032468), zMEK2b (NP_001121753), hMEK2 (NP_109587), mMEK2 (NP_075627), hMEK3 (NP_659731), mMEK3 (NP_032954), zMEK4A (NP_991299), zMEK4B (NP_001082890), hMEK4 (AAH60764), mMEK4 (NP_033183), zMEK5 (NP_001107789), hMEK5 (AAA96146), mMEK5 (BAA82040), zMEK6 (NP_571799), hMEK6 (AAB05035), mMEK6 (AAH75652), zMEK7 (XP_689405), hMEK7 (O14733), mMEK7 (Q8CE90). b. Protein sequence alignment of zebrafish MEK1 and MEK2 with those of the human and mouse. The predicted protein kinase domain is indicated. The amino acid sequence of zebrafish MEK1 showed high conservation with human (88 % identity and 94 % similarity) and mouse MEK1. Zebrafish MEK1 shared 76 % identity and 85 % similarity in the protein sequence with MEK2. Mutation sites of the constitutively activated (S219D) and dominant negative (S223A) forms of zebrafish MEK1 and MEK2 are indicated. Accession numbers for these sequences are as follows: zMEK1 (NP_998584), hMEK1 (NP_002746), mMEK1 (NP_032953), zMEK2 (zMEK2a, NP_001032468), zMEK2b (NP_001121753), hMEK2 (NP_109587), mMEK2 (NP_075627)